Conversion of the Pathogenic Fungus Colletotrichum magna to a Nonpathogenic, Endophytic Mutualist by Gene Disruption
Hygromycin-resistant transformants of the cucurbit pathogen Colletotrichum magna (teleomorph: Glomerella magna) were generated by restriction enzyme-mediated integration (REMI) transformation.A rapid pathogenicity assay involving watermelon (Citrullus lanatus) seedlings was developed and 14,400 REMI transformants were screened and assessed for thei